Functional profiling of adenylation domains in nonribosomal peptide synthetases by competitive activity-based protein profiling.
نویسندگان
چکیده
We describe competitive activity-based protein profiling (ABPP) to accelerate the functional prediction and assessment of adenylation (A) domains in nonribosomal peptide synthetases (NRPSs) in proteomic environments. Using a library of sulfamoyloxy-linked aminoacyl-AMP analogs, the competitive ABPP technique offers a simple and rapid assay system for adenylating enzymes and provides insight into enzyme substrate candidates and enzyme active-site architecture.
منابع مشابه
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عنوان ژورنال:
- Chemical communications
دوره 51 87 شماره
صفحات -
تاریخ انتشار 2015